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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > Makorin Antibody (internal region)

Makorin Antibody (internal region)

Catalog # Availability Size / Price Inquiry
APG00446G 0.1mg / $495

Makorin Antibody (internal region)

Brand

Leading Biology

Catalog Number

APG00446G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Makorin Antibody (internal region).

Molecular Weight

53349 Da

Host

Goat

Species Reactivity

Human

Isotype

IgG

Symbol

RNF61

GeneID

UniProt ID

Function

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.

Summary

This antibody may cross react with MKRN4 (52.9kDa).

Form

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, E

Images

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with APG00446G (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with APG00446G (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Specification

Quantity

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