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HMGA1 Antibody (C-term)

APR06143G Product Datasheet Protocol

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APR06143G		100 μl / $495

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Leading Biology Inc.

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HMGA1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR06143G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HMGA1 Antibody (C-term).
Molecular Weight	11676 Da
Cellular Localization	Antigen Cellular Localization: Nucleus. Chromosome.
Host	Rabbit
Species Reactivity	Human
Immunogen	64-93 aa
Target	This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.
Isotype	Rabbit Ig
Symbol	HMGIY
GeneID	3159
UniProt ID	P17096
Function	HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Summary	HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, IF, E
Dilution	WB~~1:1000 IF~~1:10~50 FC~~1:10~50
Images	Anti-HMGA1 Antibody (C-term) at 1:1000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term)( APR06143G).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).HMGA1 immunoreactivity is localized to Nucleus significantly. HMGA1 Antibody (C-term) (Cat. APR06143G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).
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