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MEK2 (MAP2K2) Antibody (N-term)

APR06042G Product Datasheet Protocol

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APR06042G		100 μl / $495

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Leading Biology Inc.

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MEK2 (MAP2K2) Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR06042G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MEK2 (MAP2K2) Antibody (N-term).
Molecular Weight	44424 Da
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	1-30 aa
Target	This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK2 (MAP2K2).
Isotype	Rabbit Ig
Symbol	MEK2, MKK2, PRKMK2
GeneID	5605
UniProt ID	P36507
Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Summary	MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	WB~~1:1000 IF~~1:10~50
Images	All lanes : Anti-MEK2 (MAP2K2) Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST. MEK2 (MAP2K2) Antibody (N-term) (Cat. APR06042G) western blot analysis in Hela,MCF-7,rat C6 cell line and mouse Liver,rat Brain tissue lysates (35ug/lane).This demonstrates the MEK2 (MAP2K2) antibody detected the MEK2 (MAP2K2) protein (arrow). Fluorescent confocal image of U251 cell stained with MEK2 (MAP2K2) Antibody (N-term)( APR06042G).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 (MAP2K2) primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).MEK2 (MAP2K2) immunoreactivity is localized to Cytoplasm significantly.
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