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CD31 Antibody (C-term)

APR05914G Product Datasheet Protocol

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APR05914G		100 μl / $495

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CD31 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR05914G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD31 Antibody (C-term).
Molecular Weight	82536 Da
Cellular Localization	Antigen Cellular Localization: Isoform Long: Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells
Host	Rabbit
Species Reactivity	Human
Immunogen	690-718 aa
Target	This CD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-718 amino acids from the C-terminal region of human CD31.
Isotype	Rabbit Ig
GeneID	5175
UniProt ID	P16284
Function	Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Summary	CD31 is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, IHC-P, E
Dilution	FC~~1:25 WB~~1:1000 IHC-P~~1:10~50
Images	Overlay histogram showing THP-1 cells stained with APR05914G (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR05914G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-CD31 Antibody (C-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: human lung lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of CD31 antibody (C-term) (Cat.APR05914G) in HL60 cell line lysates (35ug/lane). CD31 (arrow) was detected using the purified Pab.
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