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MAPE Antibody (C-term)

APR05797G Product Datasheet Protocol

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APR05797G		100 μl / $495

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Leading Biology Inc.

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MAPE Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR05797G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MAPE Antibody (C-term).
Molecular Weight	57890 Da
Cellular Localization	Antigen Cellular Localization: Nucleus. Cell membrane
Host	Rabbit
Species Reactivity	Human
Immunogen	476-502 aa
Target	This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE.
Isotype	Rabbit Ig
Symbol	MAPE, OIP4
GeneID	23532
UniProt ID	P78395
Function	Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.
Summary	MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Images	All lanes : Anti-MAPE Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: human testis lysate Lane 5: human brain lysate Lane 6: human kidney lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of MAPE antibody (C-term) (Cat. APR05797G) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab. MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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