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(Mouse) Eras Antibody (N-term)

APR04773G Product Datasheet Protocol

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APR04773G		100 μl / $495

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Leading Biology Inc.

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(Mouse) Eras Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR04773G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Eras Antibody (N-term).
Molecular Weight	24321 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side
Host	Rabbit
Species Reactivity	Mouse
Target	This Mouse Eras antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from the N-terminal region of mouse Eras.
Isotype	Rabbit Ig
GeneID	353283
UniProt ID	Q7TN89
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
Summary	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, E
Dilution	FC~~1:25 WB~~1:1000
Images	Overlay histogram showing F9 cells stained with APR04773G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR04773G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Western blot analysis of lysate from mouse F9 cell line, using Eras Antibody (N-term)(APR04773G). APR04773G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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