> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > RBBP5 Antibody (C-term)Brand |
Leading Biology | Catalog Number |
APR04741G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality RBBP5 Antibody (C-term).
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Molecular Weight |
59153 Da
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Cellular Localization |
Antigen Cellular Localization:
Nucleus.
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Target |
This RBBP5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 451-485 amino acids from the C-terminal region of human RBBP5.
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Isotype |
Rabbit Ig
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Symbol |
RBQ3
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GeneID |
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UniProt ID |
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Function |
In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
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Summary |
In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IF, E
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Dilution |
IF~~1:25
WB~~1:1000
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Images |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (Human osteosarcoma cell line) cells labeling RBBP5 with APR04741G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor? 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).
Western blot analysis of lysates from MCF-7, K562 cell line and mouse spleen tissue(from left to right), using RBBP5 Antibody (C-term)(APR04741G). APR04741G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. |
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Specification |
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Quantity |
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| Select | Brand | Catalog No. | Product Name | Pack Size | Type | Field of Research | Specification | Quantity | Price(USD) | |
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| 6 | Leading Biology | APR03502G | IGHG1 Antibody (Center) | 100 μl | Polyclonal Antibodies |
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Leading Biology Inc.
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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