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CD34 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR04736G 100 μl / $495

CD34 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR04736G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD34 Antibody (C-term).

Molecular Weight

40716 Da

Cellular Localization

Antigen Cellular Localization: Membrane; Single-pass type I membrane protein

Host

Rabbit

Species Reactivity

Human

Target

This CD34 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 320-354 amino acids from the C-terminal region of human CD34.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Summary

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, E

Dilution

IF~~1:25 WB~~1:1000

Images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized human haemangioma tissue labeling Pdx1 with APR04736G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human haemangioma tissue. The nuclear counter stain is DAPI (blue).

Western blot analysis of lysate from KG-1 cell line, using CD34 Antibody (C-term)(APR04736G). APR04736G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Specification

Quantity

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