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Mouse Pbk Antibody(N-term)

APR04430G Product Datasheet Protocol

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APR04430G		100 μl / $495

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Leading Biology Inc.

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Mouse Pbk Antibody(N-term)

Brand	Leading Biology	Catalog Number	APR04430G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Mouse Pbk Antibody(N-term).
Molecular Weight	36745 Da
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	58-86 aa
Target	This Mouse Pbk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of mouse Pbk.
Isotype	Rabbit Ig
Symbol	Topk
GeneID	52033
UniProt ID	Q9JJ78
Function	Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).
Summary	Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	FC~~1:25 IHC-P~~1:25 WB~~1:1000
Images	Overlay histogram showing A431 cells stained with APR04430G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR04430G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term)( APR04430G). APR04430G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Western blot analysis of lysate from Hela cell line, using Mouse Pbk Antibody (N-term)(APR04430G). APR04430G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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