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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > ACTA1/alpha-actin Antibody (C-term)

ACTA1/alpha-actin Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR04217G 100 μl / $495

ACTA1/alpha-actin Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR04217G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ACTA1/alpha-actin Antibody (C-term).

Molecular Weight

42051 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm, cytoskeleton.

Host

Rabbit

Species Reactivity

Human, Rat

Immunogen

189-217 aa

Target

This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the C-terminal region of human ACTA1/Alpha-actin.

Isotype

Rabbit Ig

Symbol

ACTA

GeneID

UniProt ID

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Summary

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IHC-P, WB, E

Dilution

IHC-P~~1:25 WB~~1:1000

Images

APR04217G staining ACTA1/alpha-actin in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

ACTA1/α-actin Antibody (C-term) (Cat. APR04217G) western blot analysis in human normal uterus and placenta,rat heart tissue lysates (35ug/lane).This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow).

Specification

Quantity

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