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CTBP1 Antibody (C-term)

APR04058G Product Datasheet Protocol

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APR04058G		100 μl / $495

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Leading Biology Inc.

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CTBP1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR04058G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CTBP1 Antibody (C-term).
Molecular Weight	47535 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Nucleus
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	413-440 aa
Target	This CTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human CTBP1.
Isotype	Rabbit Ig
Symbol	CTBP
GeneID	1487
UniProt ID	Q13363
Function	Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Summary	This gene encodes a protein that binds to the C-terminusof adenovirus E1A proteins. This phosphoprotein is atranscriptional repressor and may play a role during cellularproliferation. This protein and the product of a second closelyrelated gene, CTBP2, can dimerize. Both proteins can also interactwith a polycomb group protein complex which participates inregulation of gene expression during development. Alternativesplicing of transcripts from this gene results in multipletranscript variants.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	WB~~1:1000 IF~~1:10~50
Images	All lanes : Anti-CTBP1 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: mouse brain lysate Lane 6: rat brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST. CTBP1 Antibody (C-term) (Cat. APR04058G) western blot analysis in Hela,HL-60,Jurkat,Raji cell line and mouse brain,rat brain tissue lysates (35ug/lane).This demonstrates the CTBP1 antibody detected the CTBP1 protein (arrow). Fluorescent confocal image of Hela cell stained with CTBP1 Antibody (C-term)( APR04058G). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CTBP1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).CTBP1 immunoreactivity is localized to nucleus significantly.
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