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ALDH1A1 Antibody

APR03991G Product Datasheet Protocol

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APR03991G		100 μl / $495

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Leading Biology Inc.

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ALDH1A1 Antibody

Brand	Leading Biology	Catalog Number	APR03991G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALDH1A1 Antibody.
Molecular Weight	54862 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human
Target	This ALDH1A1 antibody is generated from rabbits immunized with human ALDH1A1 recombinant protein.
Isotype	Rabbit Ig
Symbol	ALDC, ALDH1, PUMB1
GeneID	216
UniProt ID	P00352
Function	Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).
Summary	ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, WB, IHC-P, FC, E
Dilution	WB~~1:1000 IF~~1:100 IHC-P~~1:50~100 FC~~1:10~50
Images	Western blot analysis of lysates from HepG2 cell line and human lung tissue lysate (from left to right), using ALDH1A1 Antibody(APR03991G). APR03991G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with APR03991G ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells. ALDH1A1 Antibody (Cat. APR03991G) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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