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MyoD1 Antibody (Center)

APR03723G Product Datasheet Protocol

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APR03723G		100 μl / $495

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Leading Biology Inc.

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MyoD1 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR03723G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MyoD1 Antibody (Center).
Molecular Weight	34501 Da
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Rabbit
Species Reactivity	Human
Immunogen	211-240 aa
Target	This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1.
Isotype	Rabbit Ig
Symbol	BHLHC1, MYF3, MYOD
GeneID	4654
UniProt ID	P15172
Function	Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Summary	This gene encodes a nuclear protein that belongs to thebasic helix-loop-helix family of transcription factors and themyogenic factors subfamily. It regulates muscle celldifferentiation by inducing cell cycle arrest, a prerequisite formyogenic initiation. The protein is also involved in muscleregeneration. It activates its own transcription which maystabilize commitment to myogenesis.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, E
Dilution	WB~~1:1000 IF~~1:10~50
Images	All lanes : Anti-MyoD1Antibody(Center) at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: RD whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST. MyoD1 Antibody (Center) (Cat. APR03723G) western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow). Fluorescent confocal image of Hela cell stained with MyoD1 Antibody (Center)( APR03723G). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MyoD1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). MyoD1 immunoreactivity is localized to nucleus significantly.
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