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KLF4 Antibody (C-term)

APR03639G Product Datasheet Protocol

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APR03639G		100 μl / $495

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Leading Biology Inc.

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KLF4 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR03639G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KLF4 Antibody (C-term).
Molecular Weight	54671 Da
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Rabbit
Species Reactivity	Human
Immunogen	394-421 aa
Target	This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-421 amino acids from the C-terminal region of human KLF4.
Isotype	Rabbit Ig
Symbol	EZF, GKLF
GeneID	9314
UniProt ID	O43474
Function	Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Summary	KLF4 is a transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, FC, E
Dilution	WB~~1:1000 IF~~1:10~50 FC~~1:10~50
Images	KLF4 Antibody (C-term) (Cat. APR03639G) western blot analysis in A431,MCF-7 cell line lysates (35ug/lane).This demonstrates the KLF4 antibody detected the KLF4 protein (arrow). Fluorescent confocal image of SY5Y cells stained with APR03639G KLF4 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with APR03639G KLF4 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (5.25 μM, 25 min). KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells. KLF4 Antibody (C-term) (Cat. APR03639G) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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