> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > MSH2 Antibody (Center)Brand |
Leading Biology | Catalog Number |
APR03581G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality MSH2 Antibody (Center).
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Molecular Weight |
104743 Da
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Cellular Localization |
Antigen Cellular Localization:
Nucleus.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
637-665 aa
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Target |
This MSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the Central region of human MSH2.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
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Summary |
MSH2 was identified as a locus frequently mutated inhereditary nonpolyposis colon cancer (HNPCC). When cloned, it wasdiscovered to be a human homolog of the E. coli mismatch repairgene mutS, consistent with the characteristic alterations inmicrosatellite sequences (RER+ phenotype) found in HNPCC. [providedby RefSeq].
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IF, FC, E
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Dilution |
WB~~1:1000
IF~~1:10~50
FC~~1:10~50
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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