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Vimentin Antibody

Catalog # Availability Size / Price Inquiry
APR06967G 100 μl / $495

Vimentin Antibody

Brand

Leading Biology

Catalog Number

APR06967G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Vimentin Antibody.

Molecular Weight

53652 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Target

This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein.

Clone

RB41034

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Summary

This gene encodes a member of the intermediate filamentfamily. Intermediate filamentents, along with microtubules andactin microfilaments, make up the cytoskeleton. The protein encodedby this gene is responsible for maintaining cell shape, integrityof the cytoplasm, and stabilizing cytoskeletal interactions. It isalso involved in the immune response, and controls the transport oflow-density lipoprotein (LDL)-derived cholesterol from a lysosometo the site of esterification. It functions as an organizer of anumber of critical proteins involved in attachment, migration, andcell signaling. Mutations in this gene causes a dominant,pulverulent cataract.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, IF, E

Dilution

WB~~1:2000 IHC-P~~1:50 FC~~1:50 IF~~1:50

Images

VIME Antibody (Cat. APR06967G) western blot analysis in Hela,U251,A549,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the VIME antibody detected the VIME protein (arrow).

Vimentin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Vimentin Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.

Specification

Quantity

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