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CLIP1 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR06132G 100 μl / $495

CLIP1 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR06132G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CLIP1 Antibody (N-term).

Molecular Weight

162246 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle. Note=Associated with the cytoskeleton. Detected at the plus ends of microtubules in the cytosol, and close to plasma membrane ruffles. Associates with the membranes of intermediate macropinocytic vesicles

Host

Rabbit

Species Reactivity

Human

Immunogen

228-254 aa

Target

This CLIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the N-terminal region of human CLIP1.

Isotype

Rabbit Ig

Symbol

CYLN1, RSN

GeneID

UniProt ID

Function

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.

Summary

CLIP1 may be a intermediate filament associated protein that links endocytic vesicles to microtubules.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Images

Anti-CLIP1 Antibody (N-term) at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 162 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

CLIP1 Antibody (N-term) (Cat. APR06132G) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLIP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CLIP1 Antibody (N-term) (Cat. APR06132G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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