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VIME Antibody

Catalog # Availability Size / Price Inquiry
APR06115G 100 μl / $495

VIME Antibody

Brand

Leading Biology

Catalog Number

APR06115G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality VIME Antibody.

Molecular Weight

53652 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Target

This Vimentin antibody is generated from rabbits immunized with Vimentin recombinant protein.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Summary

VIME is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. This protein is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50

Images

Western blot analysis of VIME Antibody (Cat. APR06115G) in A375 cell line lysates (35ug/lane). VIME (arrow) was detected using the purified Pab.

VIME Antibody (Cat. APR06115G)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VIME Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

VIME Antibody (Cat. APR06115G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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