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PRODH Antibody (Center)

APR06104G Product Datasheet Protocol

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APR06104G		100 μl / $495

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Leading Biology Inc.

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PRODH Antibody (Center)

Brand	Leading Biology	Catalog Number	APR06104G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PRODH Antibody (Center).
Molecular Weight	68036 Da
Cellular Localization	Antigen Cellular Localization: Mitochondrion matrix.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	130-155 aa
Target	This PRODH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-155 amino acids from the Central region of human PRODH.
Isotype	Rabbit Ig
Symbol	Pro1
GeneID	19125
UniProt ID	Q9WU79
Function	Converts proline to delta-1-pyrroline-5-carboxylate.
Summary	PRODH is a mitochondrial proline dehydrogenase that catalyzes the first step in proline degradation. It converts proline to delta-1-pyrroline-5-carboxylate.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, IHC-P, WB, E
Dilution	FC~~1:25 IHC-P~~1:50~100 WB~~1:1000
Images	Overlay histogram showing A549 cells stained with APR06104G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR06104G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. APR06104G staining PRODH in H. skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-PRODH Antibody (Center) at 1:2000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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