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NEK7 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR06064G 100 μl / $495

NEK7 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR06064G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NEK7 Antibody (N-term).

Molecular Weight

34551 Da

Cellular Localization

Antigen Cellular Localization: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody

Host

Rabbit

Species Reactivity

Human

Immunogen

1-30 aa

Target

This NEK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NEK7.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.

Summary

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM]

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:50~100

Images

Western blot analysis of NEK7 (arrow) using NEK7 Antibody (N-term) (Cat.APR06064G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

NEK7 Antibody (M8) (Cat. APR06064G) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NEK7 antibody detected the NEK7 protein (arrow).

Specification

Quantity

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