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CYP3A43 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR06048G 100 μl / $495

CYP3A43 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR06048G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYP3A43 Antibody (Center).

Molecular Weight

57670 Da

Cellular Localization

Antigen Cellular Localization: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein

Host

Rabbit

Species Reactivity

Human

Immunogen

242-270 aa

Target

This CYP3A43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-270 amino acids from the Central region of human CYP3A43.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Exhibits low testosterone 6-beta-hydroxylase activity.

Summary

CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, the protein unknown whether the enzyme is also involved in xenobiotic metabolism.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, FC, E

Dilution

WB~~1:2000 FC~~1:10~50 IF~~1:10~50

Images

Anti-CYP3A43 Antibody (Center) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

CYP3A43 Antibody (Center) (Cat. APR06048G) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CYP3A43 Antibody (Center)( APR06048G) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specification

Quantity

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