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TIEG Antibody (N-term)

APR06006G Product Datasheet Protocol

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APR06006G		100 μl / $495

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Leading Biology Inc.

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TIEG Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR06006G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TIEG Antibody (N-term).
Molecular Weight	52555 Da
Cellular Localization	Antigen Cellular Localization: Nucleus {ECO:0000250\|UniProtKB:O89091}.
Host	Rabbit
Species Reactivity	Human
Immunogen	13-40 aa
Target	This TIEG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TIEG.
Isotype	Rabbit Ig
Symbol	TIEG, TIEG1
GeneID	7071
UniProt ID	Q13118
Function	Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.
Summary	TIEG is a transcriptional repressor involved in the regulation of cell growth. TIEG binds to the consensus sequence 5'-GGTGTG-3'and inhibits cell growth.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:10~50
Images	All lanes : Anti-TIEG Antibody (N-term) at 1:2000 dilution Lane 1: 293T whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: human kidney lysates Lane 4: human lung lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of anti-TIEG Antibody (N-term) (Cat.APR06006G) pre-incubated with and without blocking peptide in CEM cell line lysate. TIEG(N-term)(arrow) was detected using the purified Pab . Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TIEG antibody (N-term) (Cat.APR06006G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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