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C6 Antibody (N-term)

APR05824G Product Datasheet Protocol

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APR05824G		100 μl / $495

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Leading Biology Inc.

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C6 Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR05824G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality C6 Antibody (N-term).
Molecular Weight	104786 Da
Cellular Localization	Antigen Cellular Localization: Secreted.
Host	Rabbit
Species Reactivity	Human
Immunogen	30-58 aa
Target	This C6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human C6.
Isotype	Rabbit Ig
GeneID	729
UniProt ID	P13671
Function	Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
Summary	C6 is a component of complement cascade. It is part of the membrane attack complex which can insert into the cell membrane and cause cell to lyse. People with C6 deficiency are prone to bacterial infection.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, IF, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50
Images	Western blot analysis of lysate from human blood plasma tissue lysate, using C6 Antibody (N-term)(Cat. APR05824G). APR05824G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. Formalin-fixed and paraffin-embedded human breast carcinoma reacted with C6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of MDA-231 cells using C6 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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