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OGG1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR05821G 100 μl / $495

OGG1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR05821G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality OGG1 Antibody (C-term).

Molecular Weight

38782 Da

Cellular Localization

Antigen Cellular Localization: Nucleus, nucleoplasm. Nucleus speckle. Nucleus matrix. Note=Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells Isoform 2A: Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

318-345 aa

Target

This OGG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-345 amino acids from the C-terminal region of human OGG1.

Isotype

Rabbit Ig

Symbol

MMH, MUTM, OGH1

GeneID

UniProt ID

Function

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion.

Summary

OGG1 is the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, E

Dilution

WB~~1:1000

Images

All lanes : Anti-OGG1 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NCCIT whole cell lysate Lane 6: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of OGG1 Antibody (C-term) (Cat. APR05821G) in Hela(lane 1), NIH-3T3(lane 2) cell line lysates (35ug/lane). OGG1 (arrow) was detected using the purified Pab.

Specification

Quantity

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