> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > KRT14 Antibody (Center)Brand |
Leading Biology | Catalog Number |
APR05794G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality KRT14 Antibody (Center).
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Molecular Weight |
51561 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
276-303 aa
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Target |
This KRT14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-303 amino acids from the Central region of human KRT14.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
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Summary |
KRT14 is a member of the keratin family, the most diverse group of intermediate filaments. This protein, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
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Images |
Anti-KRT14 Antibody (Center) at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human breast carcinoma with KRT14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
KRT14 Antibody (Center) (Cat.APR05794G) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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Tel: 1-661-524(LBI)-0262
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