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TAGLN Antibody (N-term)

APR05781G Product Datasheet Protocol

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APR05781G		100 μl / $495

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Leading Biology Inc.

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TAGLN Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR05781G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TAGLN Antibody (N-term).
Molecular Weight	22611 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm.
Host	Rabbit
Species Reactivity	Human
Immunogen	24-51 aa
Target	This TAGLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human TAGLN.
Isotype	Rabbit Ig
Symbol	SM22, WS3-10
GeneID	6876
UniProt ID	Q01995
Function	Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Summary	TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Images	Anti-TAGLN Antibody (N-term) at 1:8000 dilution + H. uterus whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of TAGLN antibody (N-term) (Cat. APR05781G) in SK-Br-3 cell line lysates (35ug/lane). TAGLN (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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