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REG3G Antibody (Center)

APR05660G Product Datasheet Protocol

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APR05660G		100 μl / $495

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Leading Biology Inc.

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REG3G Antibody (Center)

Brand	Leading Biology	Catalog Number	APR05660G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality REG3G Antibody (Center).
Molecular Weight	19330 Da
Cellular Localization	Antigen Cellular Localization: Secreted. Cytoplasm.
Host	Rabbit
Species Reactivity	Human
Immunogen	89-116 aa
Target	This REG3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-116 amino acids from the Central region of human REG3G.
Isotype	Rabbit Ig
Symbol	PAP1B
GeneID	130120
UniProt ID	Q6UW15
Function	Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
Summary	REG3G might be a stress protein involved in the control of bacterial proliferation (By similarity).
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	IHC-P~~1:50~100 WB~~1:1000 FC~~1:10~50
Images	AP5606C staining REG3G in human small intestine sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. AP5606C staining REG3G in human small intestine sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-REG3G Antibody (Center) at 1:2000 dilution + human pancreas lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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