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PYHIN1 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR05643G 100 μl / $495

PYHIN1 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR05643G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PYHIN1 Antibody (N-term).

Molecular Weight

55065 Da

Cellular Localization

Antigen Cellular Localization: Isoform 1: Nucleus, nucleoplasm. Isoform 5: Nucleus. Nucleus speckle.

Host

Rabbit

Species Reactivity

Human

Immunogen

26-55 aa

Target

This PYHIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human PYHIN1.

Isotype

Rabbit Ig

Symbol

IFIX

GeneID

UniProt ID

Function

Major mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization. Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells.

Summary

PYHIN1 belongs to the HIN200 family ofinterferon-inducible proteins that share a 200-amino acid signaturemotif at their C-terminal ends. HIN200 proteins are primarilynuclear and are involved in transcriptional regulation of genesimportant for cell cycle control, differentiation, and apoptosis(Ding et al., 2006 [PubMed 16479015]).

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Images

PYHIN1 Antibody (N-term)(Cat. APR05643G) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PYHIN1 antibody detected the PYHIN1 protein (arrow).

PYHIN1 Antibody (N-term) (Cat. APR05643G) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYHIN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

PYHIN1 Antibody (N-term) (Cat. APR05643G) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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