> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > NDC80 Antibody (C-Term)Brand |
Leading Biology | Catalog Number |
APR04993G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NDC80 Antibody (C-Term).
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Molecular Weight |
73913 Da
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Cellular Localization |
Antigen Cellular Localization:
Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore
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Host |
Rabbit
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Species Reactivity |
Human
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Target |
This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 508-539 amino acids from human NDC80.
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Isotype |
Rabbit Ig
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Symbol |
HEC, HEC1, KNTC2
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GeneID |
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UniProt ID |
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Function |
Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
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Summary |
Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, E
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Dilution |
WB~~1:2000
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Images |
All lanes : Anti-NDC80 Antibody (C-Term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lane 4: U-2 OS whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Specification |
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Quantity |
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Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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