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PSMA5 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR03965G 100 μl / $495

PSMA5 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR03965G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PSMA5 Antibody (Center).

Molecular Weight

26411 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

106-135 aa

Target

This PSMA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the Central region of human PSMA5.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Summary

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Images

Western blot analysis of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody (Center) (Cat.APR03965G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).

Western blot analysis of PSMA5 Antibody (Center) (Cat.APR03965G) in HL60 cell line lysates (35ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody (Center) (Cat.APR03965G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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