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H Cadherin (CDH13) Antibody (C-term)

APR03950G Product Datasheet Protocol

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APR03950G		100 μl / $495

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Leading Biology Inc.

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H Cadherin (CDH13) Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR03950G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality H Cadherin (CDH13) Antibody (C-term).
Molecular Weight	78287 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor.
Host	Rabbit
Species Reactivity	Human
Immunogen	589-617 aa
Target	This H Cadherin (CDH13) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 589-617 amino acids from the C-terminal region of human H Cadherin (CDH13).
Isotype	Rabbit Ig
Symbol	CDHH
GeneID	1012
UniProt ID	P55290
Function	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.
Summary	CDH13 is a member of the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
Images	Western blot analysis of lysate from human brain tissue lysate, using CDH13 Antibody (C-term)(Cat. APR03950G). APR03950G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDH13 antibody (C-term) (Cat.APR03950G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of MCF-7 cells using H Cadherin (CDH13) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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