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CDKN1A Antibody (C-term)

APR03821G Product Datasheet Protocol

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APR03821G		100 μl / $495

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Leading Biology Inc.

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CDKN1A Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR03821G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CDKN1A Antibody (C-term).
Molecular Weight	18119 Da
Cellular Localization	Antigen Cellular Localization: Cytoplasm. Nucleus.
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	117-146 aa
Target	This CDKN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human CDKN1A.
Isotype	Rabbit Ig
Symbol	CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WA
GeneID	1026
UniProt ID	P38936
Function	May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Summary	This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50
Images	Fluorescent image of A549 cell stained with CDKN1A Antibody (C-term)( APR03821G).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1A primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).CDKN1A immunoreactivity is localized to Nucleus significantly. CDKN1A Antibody (C-term) (Cat. APR03821G) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow). CDKN1A Antibody (C-term) (Cat. APR03821G) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow).
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