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RPL15 Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APR03737G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality RPL15 Antibody (N-term).
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Molecular Weight |
24146 Da
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
21-50 aa
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Target |
This RPL15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human RPL15.
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Isotype |
Rabbit Ig
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Symbol |
EC45
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GeneID |
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UniProt ID |
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Summary |
Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a ribosomalprotein that is a component of the 60S subunit. The protein belongsto the L15E family of ribosomal proteins. It is located in thecytoplasm. This gene shares sequence similarity with the yeastribosomal protein YL10 gene. Although this gene has been referredto as RPL10, its official symbol is RPL15. This gene has been shownto be overexpressed in some esophageal tumors compared to normalmatched tissues. Transcript variants utilizing alternative polyAsignals exist. As is typical for genes encoding ribosomal proteins,there are multiple processed pseudogenes of this gene dispersedthrough the genome.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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Images |
Anti-RPL15 Antibody (N-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST. RPL15 Antibody (N-term) (Cat. APR03737G)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. RPL15 Antibody (N-term) (Cat. APR03737G) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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