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CREB1 Antibody (Center)

APR03604G Product Datasheet Protocol

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APR03604G		100 μl / $495

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Leading Biology Inc.

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CREB1 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR03604G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CREB1 Antibody (Center).
Molecular Weight	36688 Da
Cellular Localization	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00312, ECO:0000255\|PROSITE-ProRule:PRU00978, ECO:0000269\|PubMed:12552083}
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	105-132 aa
Target	This CREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-132 amino acids from the Central region of human CREB1.
Isotype	Rabbit Ig
GeneID	1385
UniProt ID	P16220
Function	Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Summary	This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, FC, E
Dilution	WB~~1:1000 IF~~1:10~50 FC~~1:10~50
Images	All lanes : Anti-CREB1 Antibody (Center) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: C6 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-CREB1 Antibody (Center) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: C6 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent image of A549 cell stained with CREB1 Antibody (Center)( APR03604G).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CREB1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).CREB1 immunoreactivity is localized to Nucleus significantly.
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