> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > CCNB1 Antibody (C-term)Brand |
Leading Biology | Catalog Number |
APR03527G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CCNB1 Antibody (C-term).
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Molecular Weight |
48337 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
381-411 aa
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Target |
This CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-411 amino acids from the C-terminal region of human CCNB1.
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Isotype |
Rabbit Ig
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Symbol |
CCNB
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GeneID |
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UniProt ID |
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Function |
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
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Summary |
The protein encoded by this gene is a regulatory proteininvolved in mitosis. The gene product complexes with p34(cdc2) toform the maturation-promoting factor (MPF). Two alternativetranscripts have been found, a constitutively expressed transcriptand a cell cycle-regulated transcript, that is expressedpredominantly during G2/M phase. The different transcripts resultfrom the use of alternate transcription initiation sites. [providedby RefSeq].
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
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Images |
Anti-CCNB1 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
CCNB1 Antibody (C-term) (Cat. APR03527G) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CCNB1 antibody detected the CCNB1 protein (arrow).
CCNB1 Antibody (C-term) (Cat. APR03527G)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCNB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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