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Location: Home > Custom Services > Protein Related Services > Whole cell-based protein expression system

Whole cell-based protein expression system

Date: 2018-08-28 Author: Leading Biology Click: 1836

Introduction

The cell-based membrane protein expression system provided by our company is based on the transfection of recombinant target protein expression plasmids. Our expression platform includes all kinds of protein expression sources, including bacteria, yeast, insect cells and mammalian cell lines. Customers only need to provide the information of related target proteins (gene sequence, amino acid sequence, source, protein solubility, etc.). We can start from the first step of downstream protein expression, and perform vector construction, expression, purification, stabilization, even the crystallization of the membrane protein. Under the efforts of our excellent R & D team, we have developed a series of new reagents that could inhibit the degradation, maintain the full-length, natural conformation and the maximum bioactivity of the protein, we also do optimization according to different target proteins. Our customized membrane proteins can meet the requirements of downstream antibody screening, drug discovery, structural determination, function and mechanism research.


Comparison and summary

Category

Expression Time

Yield

growth medium

protein folding

post-translational modification

Bacterial

Moderately rapid

High

Simple, inexpensive

Refolding may be required

No

Yeast Cells

Moderately rapid

High

Simple, inexpensive

Refolding may be required

Yes

Insect Cells

Fairly slow

Moderate

Complex, expensive

Proper folding

Yes

Mammalian cells

Fairly slow

Moderate

Complex, expensive

Proper folding

Yes


Related Services

Protein Expression in Bacteria

Unique Protein Production Service

Protein Expression in Cell-Free System

Rich in Disulfide Bond Protein Expression

Protein Expression in Mammalian Cells

Protein Expression in Baculovirus

Protein Expression in Yeast

Protein Expression in Bacillus subtilis

Protein Expression in E coli

Virus-like particles/Phage library expression system based

Cell-free expression system

Whole cell-based protein expression system


Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

  • Innovative configurations of chromatography columns custom tailored to each protein
  • Vigorous quality control system to ensure the required quality and reproducibility
  • Production capacity of up to tens of grams
  • Flexible scale-up protein production
  • Competitive price with fast turnaround time


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

Order Products: Order Related Products

By Phone: 1-661-524(LBI)-0262 (USA)


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