Brief Introduction Of Protein Acetylation

Acetylation refers to a reaction that introduces an acetyl functional group into a chemical compound, in which the hydrogen atom of a hydroxyl group is replaced by an acetyl group (CH3 CO) to yield a specific ester, the acetate. Protein acetylation commonly has two different forms. In humans, almost (80%-90%) proteins become co-translationally acetylated at their Nα-termini of the nascent polypeptide chains. Another type is typically acetylated on lysine residues.

Protein acetylation plays a crucial role in regulating transcriptional activity. Acetylation complexes (such as CBP/p300) or deacetylation complexes (such as HDAC) can be recruited to DNA-bound transcription factors (TF) in response to signaling pathways. Histone hyper-acetylation by histone acetyl-transferases (HATs) is associated with transcriptional activation, presumably by remodeling nucleosomal structure into an open conformation more accessible to transcription complexes. Conversely, histone de-acetylation is associated with transcriptional repression reversing the chromatin remodeling process. Several transcriptional co-activators and co-repressors possess intrinsic acetylase or deacetylase enzymatic activities, respectively. Site-specific acetylation of a growing list of non-histone proteins, including p53 and E2F, has been shown to play an important role in transcriptional regulation and cell proliferation.

Our Advantages

• Quick and efficient

• Captures histone H4 acetylated at any lysine site with. extremely low detection limit

• The control is conveniently included for the quantification of the amount of acetylated histone H4

• Simple, reliable, and consistent assay conditions.

Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

• Innovative configurations of chromatography columns custom tailored to each protein
• Vigorous quality control system to ensure the required quality and reproducibility
• Production capacity of up to tens of grams
• Flexible scale-up protein production
• Competitive price with fast turnaround time

Contact Information

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