Phosphorylation

Phosphorylation is an important post-translational modification (PTM) that affects protein’s shape, intracellular behavior and function. Kinase and phosphatase remove or add phosphate groups, mainly serine, threonine and tyrosine residues. These amino acids all contain a nucleophilic group that reacts with adenosine triphosphate (ATP), replacing the oxygen on the terminal phosphorus and ejecting adenosine diphosphate (ADP).

Although kinases and phosphatases work in all living cells, they are particularly active in eukaryotes, where phosphorylation is one of about six post-translational modifications. Approximately 30% of proteins in eukaryotic cells undergo phosphorylation, which means that there are huge, untapped pathways for pharmacological intervention in almost all serious human diseases.

For pharmaceutical companies that produce about 30 approved kinase inhibitor drugs, this idea has not been lost. Small molecule tyrosine kinase inhibitors include imatinib (used to treat leukemia) and gefinitib (used to treat leukemia), and gefitinib (used for breast cancer and lung cancer) can block signals that promote cancer cell proliferation.

Determining the degree and location of phosphorylation is essential for understanding the biochemical pathways in cells, especially how to activate or inhibit cell activity. Through phosphorylation, cells regulate growth, apoptosis, cell cycle progression, and signal transduction. In this way, the continuous addition and removal of phosphates from proteins everywhere becomes a specific on/off switch for individual cell operations and for the entire cell.

Researchers use a variety of techniques to detect and quantify protein phosphorylation, including kinase activity assays, phosphorylation-specific antibodies, Western blots, enzyme-linked immunosorbent assay (ELISA), cell-based ELISA, intracellular flow cytometry, and mass spectrometry And multi-analyte analysis.

Workflow of our Phosphorylation analysis service

• In gel or in solution digestion of proteins (depends on type of samples you send us)
• Concentration of phosphor-peptides (optional; depends on the abundance of phosphor-peptide)
• HPLC separation, followed by MALDI-TOF MS/MS analysis
• Mass spectrometry data interpretation

Ordering Procedure

Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

· Innovative configurations of chromatography columns custom-tailored to each protein

· Vigorous quality control system to ensure the required quality and reproducibility

· Production capacity of up to tens of grams

· Flexible scale-up protein production

· Competitive price with fast turnaround time

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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By Phone: 1-661-524(LBI)-0262 (USA)