Introduction
An amplicon is a piece of DNA that is the product of PCR amplification of short hypervariable regions of conserved genes or intergenic regions. Because conventional analysis techniques miss the information of non-cultivable microorganisms, analysis of the amplicons using next-generation sequencing (NGS) technologies has emerged as a relatively inexpensive and fast tool for studying the diversity of microbial communities in various environmental conditions.
16S rRNA Sequencing
18S rRNA/ITS Sequencing
18S rRNA and ITS Sequencing is to amplify eukaryotes broadly with a focus on microbial eukaryotic lineages. The outlines of the method are the same as the 16S method, but different primers, PCR conditions, and sequencing primers are used. In addition, a blocking primer was designed which reduces the amplification of vertebrate host DNA, it could be used on host-associated samples, especially those that have a low eukaryotic biomass.
No | Headline | Click | Author | Date |
1 | Plant and Animal Whole Genome Re-Sequencing | 829 | Leading Biology | 2018-01-26 |
2 | Whole Exome Sequencing | 820 | Leading Biology | 2018-01-26 |
3 | Whole Transcriptome Shotgun Sequencing | 1126 | Leading Biology | 2018-01-26 |
4 | smallRNA/microRNA/circRNA/LncRNA Sequencing | 805 | Leading Biology | 2018-01-26 |
5 | Bacterial Genome Sequencing | 815 | Leading Biology | 2018-01-26 |
6 | Targeted Gene Sequencing | 912 | Leading Biology | 2018-01-26 |