16S/18S/ITS Amplicon Sequencing_Sequencing and Chip Technology_Custom Services

Introduction

An amplicon is a piece of DNA that is the product of PCR amplification of short hypervariable regions of conserved genes or intergenic regions. Because conventional analysis techniques miss the information of non-cultivable microorganisms, analysis of the amplicons using next-generation sequencing (NGS) technologies has emerged as a relatively inexpensive and fast tool for studying the diversity of microbial communities in various environmental conditions.

16S rRNA Sequencing

16S rRNA gene sequencing is a well-established method for studying phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult or impossible to study. With the ability to combine many samples in a sequencing run, microbiology researchers can use NGS-based 16S rRNA sequencing as a cost-effective technique to identify strains that may not be found using other methods.

16S rRNA Sequencing

18S rRNA/ITS Sequencing

18S rRNA and ITS Sequencing is to amplify eukaryotes broadly with a focus on microbial eukaryotic lineages. The outlines of the method are the same as the 16S method, but different primers, PCR conditions, and sequencing primers are used. In addition, a blocking primer was designed which reduces the amplification of vertebrate host DNA, it could be used on host-associated samples, especially those that have a low eukaryotic biomass.

18S rRNA/ITS Sequencing

No	Headline	Click	Author	Date
1	Plant and Animal Whole Genome Re-Sequencing	967	Leading Biology	2018-01-26
2	Whole Exome Sequencing	997	Leading Biology	2018-01-26
3	Whole Transcriptome Shotgun Sequencing	1487	Leading Biology	2018-01-26
4	smallRNA/microRNA/circRNA/LncRNA Sequencing	960	Leading Biology	2018-01-26
5	Bacterial Genome Sequencing	1040	Leading Biology	2018-01-26
6	Targeted Gene Sequencing	1104	Leading Biology	2018-01-26

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16S/18S/ITS Amplicon Sequencing

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