Targeted Gene Sequencing _Sequencing and Chip Technology_Custom Services

Introduction

Targeted gene sequencing panels are useful tools for analyzing specific mutations in a given sample. With targeted resequencing, a subset of genes or regions of the genome are isolated and sequenced. Targeted approaches using NGS allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome, specific genes of interest (custom content), targets within genes, or mitochondrial DNA. Targeted gene sequencing also produces a smaller, more manageable data set compared to broader approaches such as whole-genome sequencing, making analysis easier.

There are two methods for the targeted gene sequencing: target enrichment and amplicon generation. In the target enrichment method, Regions of interest are captured by hybridization to biotinylated probes and then isolated by magnetic pulldown, while in amplicon generation, regions of interest are amplified and purified using highly multiplexed oligo pools.

The difference between targeted sequencing and whole-genome sequencing is like below:

Targeted Gene Sequencing

No	Headline	Click	Author	Date
1	Plant and Animal Whole Genome Re-Sequencing	967	Leading Biology	2018-01-26
2	Whole Exome Sequencing	997	Leading Biology	2018-01-26
3	Whole Transcriptome Shotgun Sequencing	1487	Leading Biology	2018-01-26
4	smallRNA/microRNA/circRNA/LncRNA Sequencing	960	Leading Biology	2018-01-26
5	Bacterial Genome Sequencing	1040	Leading Biology	2018-01-26
6	Targeted Gene Sequencing	1103	Leading Biology	2018-01-26

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