Whole Transcriptome Shotgun Sequencing _Sequencing and Chip Technology_Custom Services

Introduction

Whole Transcriptome Shotgun Sequencing (WTSS), also called RNA sequencing, uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time.

WTSS including a wide variety of applications from simple mRNA profiling to discovery and analysis of the entire transcriptome, including both coding mRNA and non-coding RNA (e.g., miRNA, small RNAs, linc RNAs). These applications are extremely popular for NGS platforms as they uncover information that may be missed by array-based platforms, as no prior knowledge of the transcript sequence is needed. Also, as it is sequencing-based, it is well suited for specialty applications such as RNA editing and allele-specific expression. While there are a variety of RNA-Seq applications and protocols, most follow the basic strategy of isolating RNA (such as with poly dT to pull down mRNA), converting it to DNA and then adding adaptor sequences to generate a library suitable for sequencing.

The WTSS procedures are as follows:

Whole Transcriptome Shotgun Sequencing

No	Headline	Click	Author	Date
1	Plant and Animal Whole Genome Re-Sequencing	967	Leading Biology	2018-01-26
2	Whole Exome Sequencing	997	Leading Biology	2018-01-26
3	Whole Transcriptome Shotgun Sequencing	1487	Leading Biology	2018-01-26
4	smallRNA/microRNA/circRNA/LncRNA Sequencing	960	Leading Biology	2018-01-26
5	Bacterial Genome Sequencing	1040	Leading Biology	2018-01-26
6	Targeted Gene Sequencing	1104	Leading Biology	2018-01-26

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