Plasmids DNA Sequencing_Sequencing and Chip Technology_Custom Services

Introduction

DNA sequencing provides the most complete characterization of recombinant plasmid DNAs. Using primers targeting the plasmid backbone or the insert sequence, the identity and order of nucleotide bases for any given DNA can be determined. In the context of cloning, sequencing allows users to confirm the DNA sequence of the insert, insert orientation, and to examine the junctions between the plasmid and insert DNA.

Sanger sequencing technique is the most commonly used method for determining DNA sequences of recombinant plasmids. Sanger sequencing involves the use of a DNA polymerase, a primer, unlabeled deoxynucleotide triphosphates (dNTPs), and fluorescently labeled dideoxynucleotide triphosphates (ddNTPs), where each base is labeled with a unique fluorophore. Incorporation of a ddNTP into the newly synthesized strand prevents the addition of subsequent nucleotides, stopping the further elongation of the DNA molecule and resulting in a DNA product with a fluorescently labeled ddNTP at the end of the strand. The sequence of nucleotides can then be determined by separating the DNA products by size.

No	Headline	Click	Author	Date
1	Plant and Animal Whole Genome Re-Sequencing	967	Leading Biology	2018-01-26
2	Whole Exome Sequencing	997	Leading Biology	2018-01-26
3	Whole Transcriptome Shotgun Sequencing	1487	Leading Biology	2018-01-26
4	smallRNA/microRNA/circRNA/LncRNA Sequencing	960	Leading Biology	2018-01-26
5	Bacterial Genome Sequencing	1040	Leading Biology	2018-01-26
6	Targeted Gene Sequencing	1103	Leading Biology	2018-01-26

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