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Real Time PCR

Date: 2017-01-18 Author: Leading Biology Click: 874

Introduction

Quantitative Real-Time PCR (qRT PCR) technology refers to the method of instantaneously measuring the amount of specific products by continuously monitoring the change of fluorescence signal strength during PCR exponential amplification, and finally quantitatively analyzing the unknown template by standard curve.


Qualitative analysis:

Test the sample for a particular gene;


Absolute quantification:

Test the sample for a particular gene and determine the copy number of the gene in the sample;


Relative quantification:

Test the sample for a particular gene and determine the relative expression of the gene compared to a certain housekeeping gene and the control group;


☀ Customers should offer:

Samples: Please provide fresh and sufficient samples, tissue >100~200 mg, RNA or DNA template >5 μg;

Other information: Please provide objective gene sequence, name and gene accession number, etc.; primer sequences or primers; detailed background information; sample source and its abundance, related literature, etc.;


☀ Deliver the finished products:

The finished products delivered to customers are experimental materials such as designing synthetic fluorescent-labeled primer probes;


☀ Delivery reports:

The final delivery reports include the detailed experimental procedures, complete experimental reports, standard curves, amplification curves, dissolving curves as well as software analysis results.





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