Chemical synthesis of miRNA_Sequencing and Chip Technology_Custom Services

Introduction

In recent years, artificially synthesized miRNAs (amiRNAs) have been successfully applied to the expression of silent target genes and their functional studies. And they can specifically silence a single gene, and simultaneously silence multiple related but different genes. miRNA products mainly include miRNA mimics and miRNA inhibitors.

First of all, miRNA mimics can mimic the high level expression of endogenous mature miRNAs in cells, and further enhance the silencing of them. Besides, it can also reduce the amount of intracellular protein expression, and be used to perform gain-of-function studies.

What’s more, miRNA inhibitors can specifically target and knock out single miRNA molecule, attenuate the gene silencing effect of endogenous miRNAs, and thus it increases protein expression, and helps to carry out loss-of-function studies, in which researchers can screen miRNA target sites, and screen of miRNAs that regulate the expression of a gene and the ones affecting cell development.

Service name	Specifications （OD）	Purification method	Price	cycle
miRNA mimics (single)	2	HPLC	inquiry	1-2weeks
miRNA mimics (double)	4	HPLC	inquiry	1-2weeks
miRNA inhibitor (single)	2	HPLC	inquiry	1-2weeks
miRNA inhibitor (double)	4	HPLC	inquiry	1-2weeks
miRNA mimics (negative control)	1	HPLC	inquiry	3-5working days
miRNA inhibitor (negative control)	1	HPLC	inquiry	3-5working days

❀ Customers should offer:

Please provide objective gene sequence, GeneID or gene accession number, etc.

❀ Deliver the finished products:

Effective miRNA fragment freeze-dried powder (stored in 1.5 mL cryotubes), and it can be dispensed according to customers requirements;

❀ Delivery reports:

Sequences, concentrations, OD, the exact number of nanomolar numbers and purification methods.etc.

❀ Remarks:

It is recommended to be kept in -20℃ to preserve and try to avoid repeated freezing and thawing.

No	Headline	Click	Author	Date
1	Plant and Animal Whole Genome Re-Sequencing	967	Leading Biology	2018-01-26
2	Whole Exome Sequencing	997	Leading Biology	2018-01-26
3	Whole Transcriptome Shotgun Sequencing	1487	Leading Biology	2018-01-26
4	smallRNA/microRNA/circRNA/LncRNA Sequencing	960	Leading Biology	2018-01-26
5	Bacterial Genome Sequencing	1040	Leading Biology	2018-01-26
6	Targeted Gene Sequencing	1103	Leading Biology	2018-01-26

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