ScFv Phage Library Construction Service

Human scFv Antibody Libraries

Phage display antibody libraries are widely used in screening high affinity antibodies, such as screening of angiogenesis markers fibronectin, tumor-specific B3/B4 antigen and EGFR. Antibody libraries constructed by phage display techniques are usually derived from RT-PCR amplification of peripheral blood mononuclear cells, spleen cells, bone marrow cells and tonsil cells mRNAs to form cDNA fragments. These gene fragments were spliced into phagemid vectors, and then transferred into phages to form covalent structural protein and display in vitro. 

The scFv antibody library refers to the variable region (VH and VL), especially the CDR region genes of the antibody, forms a large number of different clones by different strategies, such as amino acid mutations, frameshift mutations. And high affinity single chain Fv antibodies against the target antigen were screened from these clones. Since the scFv antibody has a smaller molecular weight (25 kDa), the scFV antibody is more stable and does not easily form a multimer compared with the Fab antibody library.

Types of Phage Display System

Leading Biology has extensive experience in phage display platform by employing various types of phage in various project objectives. In general, M13 phage system is the most popular vehicle choice for phage display and extensively used in many types of research. We also provide phage display related services via either T4 or T7 phage systems, which can provide different promising alternatives by their special merits. Meanwhile, clients can also provide antigens, then higher affinity and specificity of monoclonal antibodies can be obtained by sensitizing peripheral blood lymphocytes in vitro.

Our services: 

Phage display is a laboratory technique that uses bacteriophages to associate proteins with their respective genetic information for studying protein–protein, protein–peptide, and protein–DNA interactions. Our scientists have applied this technology to display antibodies for therapeutic protein engineering. Our phage display services allow the selection (“panning”) of recombinant antibody fragments from antibody gene libraries and screening of individually selected antibody clones more convenient and more specific.

Our scFv phage library construction services include:

Construction of scFv phage library using B cells from immunized or naive donors:

Leading Biology designs appropriate primers to PCR amplify VH and VL fragments (antibody variable regions of the heavy and light chains) of immunized or naive donors from several species, links them with a short linker peptide of 10-25 amino acids to generate scFv, and subsequently subclone the scFv library into our engineered phagemid vector.

Optimization of scFv phage library: 

Leading Biology uses various mutagenesis strategies to generate a library of your specific scFv mutants for affinity maturation or other optimization purposes.

Key Features

§ Various types of pre-madeantibody libraries (immune, naive or synthetic library)

§ Large library capacity (107 to 1012) and high diversity (2.0×109)

§ Screening for high affinity antibodies (10-7 to 10-9)

§ Various phage display systems (M13, T4, T7)

§ Different species of antibody libraries (mouse, rabbit, rat, camel and human)

And More

Leading Biology antibody libraries with rich diversity and large scale, are ideal for screening all antigen types for selection of functional antibodies with high affinity. With years of experience, we have developed several phage platforms that can customize a particular library for you. If you are interested in starting an antibody project with LBI, please contact us.

You may need other services, such as antibody sequencing, scFv / Fab, and Fc fusion proteins.