Introduction
Different from small molecular compounds, the biotech drugs may have a strong stimulation of the specific and sensitized lymphocytes when getting into the body. The anti-drug antibodies may cause adverse reactions like anaphylaxis, immunosuppression and autoimmune response of the body. The monkey source antibody has a higher homology with human antibody compare to other mammalian source antibodies, thus the study of immunology and pharmacology carried out in monkeys has higher data reliability. The anti-drug antibodies produced by monkeys for certain drugs usually could simulate the same reactions in the human body.
Based on phage display technology, we could achieve rhesus, macaque, marmoset and ateles immune organs cDNA library full spectrum construction and screening, the synthesized antibody library can guarantee high detection rates and screening ranges. At the same time, the antibody specificity and titer are higher than other rodents or rabbits antibodies. Leading Biology has a complete antibody humanization technical service, could provide technical support for further processing of human recombinant antibodies.
Service Package
|
Service Items |
Time (weeks) |
Peptide |
Small Molecule |
Phosphate/Methyl/Acetyl-specific |
||||
|
Peptide design & synthesis |
Peptide design |
-2 |
Free, Optional |
|||||
|
Peptide synthesis |
√ |
|
|
|
√ |
|
||
|
Conjugation |
√ |
|
√ |
|
√ |
|
||
|
Immunization |
Immune 1 Rhesus |
-8 |
√ |
√ |
√ |
√ |
√ |
√ |
|
ELISA detection |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
cDNA preparation |
Prepare cDNA library by RT-PCR |
-1 |
√ |
√ |
√ |
√ |
√ |
√ |
|
cDNA library QC check |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Fab library construction |
Obtained VH and VL genes |
10-15 |
√ |
√ |
√ |
√ |
√ |
√ |
|
Primary antibody library construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Fab library construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Phage screening |
Phage particles package |
10-15 |
√ |
√ |
√ |
√ |
√ |
√ |
|
Magnetic beads affinity screening |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Recover positive clones and DNA sequencing |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Antibody expression |
Synthetic of target phage gene and Fc gene |
-5 |
√ |
√ |
√ |
√ |
√ |
√ |
|
Eukaryotic system expression plasmids construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Eukaryotic system expression |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Stably transfected cell line construction |
|
|
|
|
|
|
||
|
Purification and detection |
Lable tag affinity purification |
-1 |
√ |
|
√ |
|
√ |
|
|
Antigen specific affinity purification |
√ |
|
√ |
|
√ |
|
||
|
Total lead time(months) |
7-9 |
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Note:
1. Price needs negotiation;
2. Please think carefully about the experiment period, the time listed above is only the shortest time under ideal condition;
3. To shorten the experiment time, we suggest you consider provide antigen and start directly from immunization;
4. After immunization, we would check the titer, if titer reaches standard, collect the spleen, if not, end the experiment;
5. Before the antibody library construction program start, we would charge at least 50% of the total price, after the program finished, pay off the rest amount of money.
Related Services
Mouse Monoclonal Antibody Service
Rat Monoclonal Antibody Service
Rabbit Monoclonal Antibody Production
Chicken Monoclonal Antibody Service
Monkey Monoclonal Antibody Service
Why Leading Biology
With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:
• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.
• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.
• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.
• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.
Contact Information
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)
| No | Headline | Click | Author | Date |
| 1 | Antibody Production | 2992 | Leading Biology | 2020-01-16 |
| 2 | Mouse Monoclonal Antibody preparation by phage display | 1597 | Leading Biology | 2019-11-20 |
| 3 | Rabbit Monoclonal Antibody preparation by phage display | 1968 | Leading Biology | 2019-11-20 |
| 4 | Antibody Labeling Services | 6362 | Leading Biology | 2019-11-05 |
| 5 | Custom Monospecific Antibody Production | 2314 | Leading Biology | 2019-11-04 |
| 6 | Custom Peptide Antibody Production | 7164 | Leading Biology | 2019-11-04 |