Introduction

Leading Biology dedicated itself from the first day to develop monoclonal antibodies almost exclusively. We have an onsite vivarium and working to establish a GMP facility soon. We keep a veterinarian on staff to make sure our mice and other animals are in the best health.

We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application.

Our technical staff with decades of experience in immunology, biochemistry, and molecular biology will be there to guide you through the 10 to 14-week process.

Service Package

Note:

1. Because the laboratory animals are  relatively fragile, and the preparation process is complex, to prevent experimental accidents, the number of mice for monoclonal antibody preparation project is at least five, each antigen at least immune 3 mice;

2. For monoclonal antibody services, if the customer want antigen specific affinity purification rather than normal specific affinity purification, the price increases 200$;

3. We could provide prokaryotic protein and eukaryotic protein synthesis service, including free protein structure analysis and codons optimization;

4. The prices listed above are catalogue prices, the exact price need to be decided according to the actual situation of the project;

Classic case 

(Mouse Monoclonal Antibody Service)

Customer Provide

Protein antigen\plasmid\protein information

Process

1. Antigen preparation\Plasmid expression\Protein purification\Peptide design, synthesis and conjugation.

2.  immunization of 5-6 Balb/c mice.

3. ELISA/Western-blot test to monitor the antibody titer and specificity in mice serum, according to ELISA and WB test result, screening mice.

4. cell fusion: fusing with the myeloma cells SP2/0.

5. Positive screening: Using the ELISA test to screening the antibody positive hybridoma clone.

6. Subclone: Using limited dilution to produce subclone from positive clone. According to customers’ requirements, we also could screen out the monoclonal cell line which could produce Ig antibodies firmly (extra charge).

7. Ascites preparation: Using mice celiac inoculation to produce the ascites of hybridoma products.

8. Antibody purification: Purify the secretory antibodies from 2 monoclonal hybridoma cell lines.

Products 

(each antigen, 5 mice)

1. 5mg polypeptide

2. 2 immunogen specific monoclonal hybridoma cell lines.

3. Each cell line provide 10ml (1mg/ml) purified antibody, ELISA  titer was above 1:50,000, WB test positive.

4. If customer require, we could provide: prepared ascites (titer 1:500-1000, extra charge), hybridoma cell culture supernatant (for WB test).

5. Complete lab report, remaining antigens.

Preparation Period

3-4 months

Successful Result

Publication: Acta Biochim Biophys Sin,

IF:2.191

Title: ACAT1 regulates the dynamics of free cholesterols in the plasma membrane which leads to the APP-α-processing alteration

Name: anti-ACAT1 antibody

Species: human

Type: Mouse monoclonal antibody

Related Services

Mouse Monoclonal Antibody Service

Rat Monoclonal Antibody Service

Rabbit Monoclonal Antibody Production

Chicken Monoclonal Antibody Service

Monkey Monoclonal Antibody Service

Canine Monoclonal Antibody Service

Hamster Hybridoma Production

Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

Order Products: Order Related Products

By Phone: 1-661-524(LBI)-0262 (USA)