> Custom Services > Antibody Discovery Services > Chicken Monoclonal Antibody Service
Introduction
Compared with common mammalian IgG, the chicken IgY antibody has a strong resistance to heat, acid, ionic and have certain enzyme resistance, it can be used as an ideal diagnostic reagent and a drug for prevention and treatment. The Leading Biology using SPF level chicken, ensures the pure background and higher production of the antibody, by using international advanced nano latex immuno amplification technology, acquire antigens with stronger immunogenicity, improve the immunization success possibility, and get chicken antibody with higher titer.
Service Package
|
Service Items |
Time (weeks) |
Peptide |
Small Molecule |
Phosphate/Methyl/Acetyl-specific |
||||
|
Peptide design & synthesis |
Peptide design |
-2 |
Free, Optional |
|||||
|
Peptide synthesis |
√ |
|
|
|
√ |
|
||
|
Conjugation |
√ |
|
√ |
|
√ |
|
||
|
Immunization |
Immune 2 chickens |
-8 |
√ |
√ |
√ |
√ |
√ |
√ |
|
ELISA test |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
cDNA preparation |
Prepare cDNA library by RT-PCR |
-1 |
√ |
√ |
√ |
√ |
√ |
√ |
|
cDNA library QC check |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Fab library construction |
Obtained VH and VL genes |
10-15 |
√ |
√ |
√ |
√ |
√ |
√ |
|
Primary antibody library construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Fab library construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Phage screening |
Phage particles package |
10-15 |
√ |
√ |
√ |
√ |
√ |
√ |
|
Magnetic beads affinity screening |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Recover positive clones and DNA sequencing |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Antibody expression |
Synthetic of target phage gene and Fc gene |
-5 |
√ |
√ |
√ |
√ |
√ |
√ |
|
Eukaryotic system expression plasmids construction |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Eukaryotic system expression |
√ |
√ |
√ |
√ |
√ |
√ |
||
|
Stably transfected cell line construction |
|
|
|
|
|
|
||
|
Purification and detection |
Lable tag affinity purification |
-1 |
√ |
|
√ |
|
√ |
|
|
Antigen specific affinity purification |
√ |
|
√ |
|
√ |
|
||
|
Total lead time(months) |
7-9 |
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Note:
1. Price needs negotiation;
2. Please think carefully about the experiment period, the time listed above is only the shortest time under ideal condition;
3. To shorten the experiment time, we suggest you consider provide antigen and start directly from immunization;
4. After immunization, we would check the titer, if titer reaches standard, collect the spleen, if not, end the experiment;
5. Before the antibody library construction program start, we would charge at least 50% of the total price, after the program finished, pay off the rest amount of money.
Related Services
Mouse Monoclonal Antibody Service
Rat Monoclonal Antibody Service
Rabbit Monoclonal Antibody Production
Chicken Monoclonal Antibody Service
Monkey Monoclonal Antibody Service
Why Leading Biology
With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:
Contact Information• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.
• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.
• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.
• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)
| No | Headline | Click | Author | Date |
| 1 | Antibody Production | 2992 | Leading Biology | 2020-01-16 |
| 2 | Mouse Monoclonal Antibody preparation by phage display | 1597 | Leading Biology | 2019-11-20 |
| 3 | Rabbit Monoclonal Antibody preparation by phage display | 1968 | Leading Biology | 2019-11-20 |
| 4 | Antibody Labeling Services | 6362 | Leading Biology | 2019-11-05 |
| 5 | Custom Monospecific Antibody Production | 2314 | Leading Biology | 2019-11-04 |
| 6 | Custom Peptide Antibody Production | 7164 | Leading Biology | 2019-11-04 |
Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
