Introduction

For different experimental uses of antibodies, to fully meet the customer's experimental needs, our technicians doing objective research for different purposes of each customer's experiment, trying to provide efficient and accurate antibody preparation technology services for customers with the most reliable qualities. we can not only undertake the entire service from the upstream antibody preparation to the downstream experimental application, but also provide preliminary test results, cooperate with customers' validation test, in every aspect of the experiment: technology discussion, test improvement and result validation, we'd communicate with customers timely and accurately to meet all kinds of requirements of scientific research, medicinal, industry, health and other fields.

Classic Case

Publication：Res Vet Sci.    

IF：2.191

Title：Changes in the expression of Heat Shock Proteins in ovaries from bovines with cystic ovarian disease induced by ACTH.   

Name：Anti-HSP27/HSP60/HSP70/HSP90 antibody

Type：Mouse monoclonal antibody

Species: Argentinean Holstein heifers

 

Publication：EMBO Rep              

IF：7.73

Title：ULK1 translocates to mitochondria and phosphorylates FUNDC1 to regulate mitophagy. 

Name：Anti-p-ser17 fundc1 antibody

Type：Rabbit polyclonal antibody

Species: Mouse    

Service process and package

Service Items		Time(months)	Start point
			peptide	Molecular	Phosphate/Methyl /acetyl-specific	purified protein	Expression plasmid	Prokaryotic proteins synthesis de novo	Eukaryotic proteins synthesis de novo
Peptide design & synthesis	Peptide design	0.5	Free
	Peptide synthesis		√		√
	conjugation		√	√	√
proteins preparation	cDNA synthesis	1
	Vector construction							√	√
	Protein expression & purification						√	√	√
Immunization	Immunized 2 mice	1.5	√	√	√	√	√	√	√
	Elisa detection		√	√	√	√	√	√	√
Fusion & Screening		1.5	√	√	√	√	√	√	√
Subcloning（ELISA Screening)		0.5	√	√	√	√	√	√	√
Monoclonal (IHC Screening)		1	√	√	√	√	√	√	√
Ascites production (Injection on 3 mice)		1	√	√	√	√	√	√	√
Purification and detection	Affinity purification	0.5	√	√	√	√	√	√	√
	Antigen specific affinity purification
Total lead time(months)			4~6			~4	4~6	5~7	6~8

Related Services

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Capture\Report Paired Antibody Service

Anti-isotype Antibody Production

Custom Antibody Services for Post-translational Modification

Ligand Memetics Agonistic Antibody Discovery Service

Interference Function/Anti-idiotype Antibody Service

Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
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By Phone: 1-661-524(LBI)-0262 (USA)