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Location: Home > Custom Services > Antibody Discovery Services > Hamster Hybridoma Production

Hamster Hybridoma Production

Date: 2018-08-27 Author: Leading Biology Click: 1389

Introduction

Together with Diagnostics, Leading Biology has extensive experience in producing monoclonal antibodies in hamsters. The use of hamsters as the host animal offers a unique species alternative in areas where mouse and rat antibodies may not work well. We can employ up to 10 hamsters for each antigen. Immune responses are elicited using immunogens such as transfected cell lines, DNA, recombinant proteins, native proteins, peptides or haptens conjugated to carrier molecules.


We perform splenocyte fusion on one or more animals. We select and subclone the strongly positive clones from the parental cultures by 2 rounds of limiting dilution cloning. Stable clones will be expanded into 24-well plates and then into T-flasks for further characterization. At no additional cost, clients could select one clone for in vitro production of approximately 1L of supernatant. Supernatants will be purified using Protein A/G column and pure antibodies will be deliverable. We also offer a full range of contract manufacturing services, including in vitro Antibody and Protein Production in bioreactors and large-scale Antibody Purification.

 

Our high affinity hamster antibodies are suitable for common research applications, including Chromatin Immunoprecipitation, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot.


Service package


Service Items

Time

(months)

Start point

Peptide

Small Molecule

Phosphate/Methyl/Acetyl-specific

Purified protein

Expression plasmid

Prokaryotic proteins synthesis de novo

Eukaryotic proteins synthesis de novo

Peptide design & synthesis

Peptide design

0.5

Free, Optional

 

 

 

 

Peptide synthesis

 

 

 

 

 

Conjugation

 

 

 

 

Proteins preparation

cDNA synthesis

1

 

 

 

 

 

Need further inquiry

Need further inquiry

Vector construction

 

 

 

 

 

Protein expression & purification

 

 

 

 

Immunization

Immune 3-5 Hamsters

1.5

ELISA test

Cell Fusion & Screening

1.5

Subcloning

0.5

Ascites production

(Inject 5 Hamsters)

1

Purification and detection

Affinity purification

0.5

Antigen specific affinity purification

 

 

 

 

 

 

 

Total lead time(months)

3-5

-3

3-5

4-6

4-6


Note:

1. Hamster source antibody and mouse source antibody have high similarity in their sequence, these similar sequences mostly concentrate on the Fc segment of antibodies, thus, to avoid the cross reaction with the mouse source antibody, hamster source antibody should use in combination with the secondary antibody which specifically recognize the hamster source antibody;

2. The specificity of hamster source antibody is better than mouse source, only after human source antibody;

3. Because the laboratory animals are  relatively fragile, and the preparation process is complex, to prevent experimental accidents, the number of hamsters for the monoclonal antibody preparation project is at least five;

4. We use SPF level animals in the project, and Infectious antigen is strictly prohibited, the customers should acknowledge us if there are specific problems of the antigen, if the customers hiding their antigen information deliberately, and cause significant financial losses of the company, we will reserve the right to the legal responsibility;

5. The prices listed above are for reference only, the exact price needs to be decided according to the actual situation of the project.


Classic case 

(Hamster monoclonal antibody service)

Customer Provide: Protein antigen\plasmid\protein information


Process

1. Antigen preparation\Plasmid expression\Protein purification\Peptide design, synthesis and conjugation.

2. Immunization of 2 Syrian hamsters.

3. ELISA/Western-blot test to monitor the antibody titer and specificity in hamster serum, according to ELISA and WB test result, screening hamsters.

4. Cell fusion

5. Positive screening: Using ELISA test to screening the antibody positive hybridoma clone.

6. Subclone: Using limited dilution to produce subclone from a positive clone, provide monoclonal supernatant to customers for tests.

7. Ascites preparation: Using mice celiac inoculation to produce the ascites of hybridoma products.

8. Antibody purification: Purify the secretory antibodies from 2 monoclonal hybridoma cell lines.


Products 

(each antigen, 5 hamsters)

1. 1-2 immunogen specific monoclonal hybridoma cell lines.

2. Each cell line provide 10ml (1mg/ml) purified antibody, ELISA  titer was above 1:50,000, WB test positive.

3. Complete lab report, remaining antigens.


Preparation time

3-6 months


Successful Result

Publication: Plos genetics

IF:6.66

Title: KIF7 Controls the Proliferation of Cells of the Respiratory Airway through Distinct Microtubule Dependent Mechanisms

Name: anti-Podoplanin antibody

Species: human

Type: Hamster Monoclonal antibody

Recommended secondary antibody: biotinylated anti-hamster lgG


F1. KIF7 is expressed as puncta in podoplanin+ (PDPN) type 1 AECs and fatty acid synthetase+ (FASN) type 2 AECs, the green region targeted KIF7 and the red showed PDPN location. The concentration of antibody that used for PDPN is 1:500.


Related Services

Mouse Monoclonal Antibody Service

Rat Monoclonal Antibody Service

Rabbit Monoclonal Antibody Production

Chicken Monoclonal Antibody Service

Monkey Monoclonal Antibody Service

Canine Monoclonal Antibody Service

Hamster Hybridoma Production


Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:


• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)


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